Part:BBa_J435309

DN4_GS flexible linker

A GS C terminal flexible linker. Defined as a DT4 part.

FLANKED 5' fusion site: AGGT, 3' fusion site: TTCG

Source organism: N/A, Target organism: Escherichia coli

Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003455.html

The 3' BsaI fusion site is blocked by dcm methylation. Do not propogate in DH5a or any other dcm+ strain before use in IIs assembly.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]